Faculty

K. Sandeep Prabhu

Assistant Professor of Veterinary & Biomedical Sciences
122 Agricultural Science & Industries Building
Telephone: 814-863-8976
FAX: 814-863-6140
Email: ksp4@psu.edu

Research Focus

Studies in our laboratory are focused around various cellular and molecular mechanisms by which micronutrients such as selenium alter the host response and immune function in response to oxidative stress caused by environmental toxicants and pathogens. We are interested in learning more about the role of a unique monooxygenase called MIOX in health and disease, with particular relevance to diabetes. Using the MIOX-/- mice in-vitro cell cultures, we are making an effort to understand its role as a regulator of lipid and carbohydrate metabolism. Studies are also focused around the transcriptional regulation of MIOX and the regulation of its activity by ROS.

Publications

  1. K. S. Prabhu and C. S. Ramadoss (1995) α-Chymotrypsin catalyzed hydrolysis of N-benzoyl-L-tyrosine ethyl ester anchored in β-cyclodextrin. Biocatal. Biotransform. 12: 281-291.
  2. K. S. Prabhu and C. S. Ramadoss (1998). Subtilisin catalysis of substrate anchored in cyclodextrin. Appl. Biochem. Biotechnol. 69: 69-77.
  3. T. Shivashankar, R. S. Annadurai, M. Srinivas, G. Preethi, T. B. Sharada, R. Paramashivappa, A. Srinivasa Rao, K.S. Prabhu, C. S. Ramadoss, G. K. Veeresh and P. V. Subba Rao (2000). Control of coconut black-headed caterpillar (Opisina arenosella Walker) by systemic application of “Soluneem”-a new water-soluble neem insecticide formulation. Curr. Sci (India) 78 : 176-178.
  4. K. S. Prabhu and C. S. Ramadoss (2000). Penicillin acylase catalyzed synthesis of Penicillin-G from cyclodextrin anchored substrates. Ind. J. Biochem. Biophys. 37: 6-12.
  5. R. J. Arner, K. S. Prabhu, J. T. Thompson and C. C. Reddy (2001). Myo-Inositol Oxygenase: Molecular Cloning and Expression of a Unique Enzyme that Oxidizes myo-Inositol and D-chiro-inositol. Biochem. J. 360: 313-320.
  6. F. Zamamiri-Davis, Y. Lu, J. T. Thompson, K. S. Prabhu, P. V. Reddy, L. M. Sordillo, and C. C. Reddy (2002). Nuclear factor-kappaB mediates over-expression of cyclooxygenase-2 during activation of RAW264.7 macrophages in selenium deficiency. Free Radic. Biol. Med. 32: 890-97.
  7. K. S. Prabhu, P. V. Reddy, E. Gumpricht, G. R. Hildenbrandt, R. W. Scholz and C. C. Reddy (2001). Microsomal class alpha glutathione S-transferase with glutathione peroxidase activity from sheep liver: Molecular cloning, expression and characterization Biochem. J. 360: 345-354.
  8. K. S. Nagabhushana, S. Umamaheshwari, F. E. Tocoli, K. S. Prabhu, I. R. Green, and C. S. Ramadoss (2002). Inhibition of Soybean and Potato Lipoxygenases by Bhilawanols from Bhilawan (Semecarpus anacardium) Nut Shell Liquid and some Synthetic Salicylic Acid Analogues. J. Enzyme Inhibition Med. Chem. 17: 255-259.
  9. K. S. Prabhu, F. Zamamiri-Davis, J. Stewart, J. T. Thompson, L. M. Sordillo and C. C. Reddy. (2002) Selenium deficiency increases the expression of inducible nitric oxide synthase (iNOS) in RAW264.7 macrophages: role of nuclear factor-kappa B in up-regulation. Biochem. J. 366: 203-209.
  10. P. Reddanna, K. S. Prabhu, J. Whelan and C. C. Reddy (2003) Carboxypeptidase catalyzed conversion of leukotriene C4 to leukotriene F4. Arch. Biochem. Biophys. 413: 158-163.
  11. K. S. Prabhu, Padala V. Reddy, Andrew D. Liken, Emily C. Jones, Hemant P. Yennawar, and C. Channa Reddy. (2003) Identification, characterization, and properties of a class alpha microsomal glutathione S-transferase. Adv Exp Med Biol. 525:189-92.
  12. K. S. Prabhu, P. V. Reddy, E. C. Jones and C. C. Reddy (2004) Characterization of a class Alpha glutathione S-transferase with glutathione peroxidase activity in human liver microsomes. Arch. Biochem. Biophys. 424: 72-80.
  13. R. J. Arner, K. S. Prabhu and C. C. Reddy (2004) Molecular cloning, expression, and characterization of myo-inositol oxygenase from mouse, rat, and human kidney. Biochem. Biophys. Res. Commun. 324:1386-92.
  14. K. S. Prabhu, R. J. Arner, Hema Vunta, and C. C. Reddy (2005) Up-regulation of myo-inositol oxygenase by hyperosmotic stress in renal proximal tubular epithelial cells. J. Biol. Chem. 280:19895-901.
  15. R. J. Arner, K. S. Prabhu, V. Krishnan, M. Johnson, and C. C. Reddy (2006) Expression of myo-inositol oxygenase in tissues susceptible to diabetic complications. Biochem. Biophys. Res. Commun. 339: 816-820.
  16. D. J. Kim, K. S. Prabhu, F. J. Gonzalez, and J. M. Peters (2006) Inhibition of chemically-induced skin carcinogenesis by sulindac is independent of peroxisome proliferator-activated receptor-/ (PPAR/) Carcinogenesis. 27: 1105-1112.
  17. G. Xing, Y Diao, L. M. Hoffart, E. W. Barr, K. S. Prabhu, R. J. Arner, C. C. Reddy, K. Krebs, J. M. Bollinger (2006) Evidence for C-H cleavage by an iron-superoxide complex in the glycol cleavage reaction catalyzed by myo-inositol oxygenase. Proc. Natl. Acad. Sci. (USA) 103: 6130-6135.
  18. G. Xing, L. M. Hoffart, Y. Diao, E. W. Barr, K. S. Prabhu, R. J. Arner, C. C. Reddy, K. Krebs, J. M. Bollinger (2006) A coupled dinuclear iron cluster that is perturbed by substrate binding in myo-inositol oxygenase. Biochemistry 45: 5393-5401.
  19. G. Xing, E. W. Barr, L. M. Hoffart, K. S. Prabhu, R. J. Arner, C. C. Reddy, K. Krebs, J. M. Bollinger (2006) Oxygen activation by a mixed-valent, diiron (II/III) cluster in the glycol cleavage reaction catalyzed by myo-inositol oxygenase. Biochemistry 45: 5402-5412.
  20. A. M. Mastro, K. S. Prabhu, H. Vunta, L. Novinger, and C. C. Reddy (2007) The inflammatory response and breast cancer metastasis to bone: A role for selenium? J. Nutrition.  137 (1): 294S-295S.
  21. J. Coleman, K. S. Prabhu, J.T. Thompson, P. S. Reddy, B. R. Peterson, J. M. Peters, C. C. Reddy, and J. P. Vanden Heuvel (2007) The oxidative stress mediator 4-hydroxynonenal is an intracellular agonist of the nuclear receptor peroxisome proliferator-activated receptor-β/δ. Free Radic. Biol. Med. 42: 1155-1164.
  22. Vunta, H, F. Davis, U. Palempalli, R. J. Arner, D. G. Peterson, C. C. Reddy, and K. S. Prabhu (2007) The anti-inflammatory activity of selenium is mediated through 15d-prostaglandin J2 in macrophages. J. Biol. Chem. Apr 17. 282: 17964-17973.
  23. P. Kalantari, H. Vunta, U. Palempalli, C. C. Reddy, A. J. Henderson, and K. S. Prabhu (2007) Nutritional control of HIV-AIDS: A case for selenium. Am. J. Infectious Diseases 3(4): 195-201.
  24. H. Vunta, B. Belda, J. P. Vanden Heuvel, and K. S. Prabhu (2007) The anti-inflammatory properties of selenium in macrophages. Mol. Nutr. Food Res. (Being revised)
  25. U. Palempalli, H. Vunta, P. Kalantari, R. J. Arner, C. C. Reddy, and K. S. Prabhu (2007) Gambogic acid covalently modifies IkBkinase-b subunit to mediate suppression of lipopolysaccharide-induced activation of NF-kB in macrophages. J. Biol. Chem. (Being revised)

 

 

 

 

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